358 .
ATYP MEDIUM
What is this page?
Equipment needed:
Filter
Final pH:
6.9
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Trace element solution SLA | 1.0 | ml | ||
|
|
Vitamin solution VA | 1.0 | ml | ||
|
|
KH2PO4 | 1.0 | g | ||
|
|
MgCl2 x 6 H2O | 0.5 | g | ||
|
|
CaCl2 x 2 H2O | 0.1 | g | ||
|
|
NH4Cl | 1.0 | g | ||
|
|
NaCl | 1.0 | g | ||
|
|
Sodium acetate | 1.0 | g | ||
|
|
Sodium succinate | 1.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
0.5 | g | ||
|
|
Bacto peptone
(BD-Difco) |
0.3 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 6.9. | |||||
| 2 Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium. | |||||
Trace element solution SLA #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
FeCl2 x 4 H2O | 1800 | mg | ||
|
|
CoCl2 x 6 H2O | 250 | mg | ||
|
|
NiCl2 x 6 H2O | 10 | mg | ||
|
|
CuCl2 x 2 H2O | 10 | mg | ||
|
|
MnCl2 x 4 H2O | 70 | mg | ||
|
|
ZnCl2 | 100 | mg | ||
|
|
H3BO3 | 500 | mg | ||
|
|
Na2MoO4 x 2 H2O | 30 | mg | ||
|
|
Na2SeO3 x 5 H2O | 10 | mg | ||
|
|
Distilled water | 1000 | ml | ||
| 1 Adjust pH to 2 - 3 with HCl. | |||||
Vitamin solution VA #
100
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 10 | mg |
|
|
Niacin | 35 | mg |
|
|
Thiamine HCl | 30 | mg |
|
|
p-Aminobenzoic acid | 20 | mg |
|
|
Pyridoxine hydrochloride | 10 | mg |
|
|
Calcium pantothenate | 10 | mg |
|
|
Vitamin B12 | 5 | mg |
|
|
Distilled water | 100 | ml |