Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
Middlebrook 7H10 agar
(BD-Difco) |
19 | g | ||
|
Glycerol | 5 | ml | ||
|
Distilled water | 895 | ml | ||
1 Sterilize at 121C for 10 min and cool to 50 - 55C. Aseptically add 100 ml of Middlebrook OADC Enrichment (BD-BBL) and mix thoroughly. |