402 .
HALOCOCCUS DOMBOWSKII MEDIUM
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Equipment needed:
Final pH:
7.4
Select a strain (optional):
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Casamino acids
(BD-Difco) |
5.0 | g | ||
|
|
Yeast extract | 5.0 | g | ||
|
|
Tris base | 12.1 | g | ||
|
|
KCl | 2.0 | g | ||
|
|
MgCl2 x 6 H2O | 20.0 | g | ||
|
|
CaCl2 x 2 H2O | 0.2 | g | ||
|
|
NaCl | 200.0 | g | ||
| 1 Add components to distilled water and bring volume to 1.0 L. | |||||
| 2 Adjust pH to 7.4. | |||||
| 3 For preparation of solid medium, add 20.0 g/L agar. | |||||
| 4 Comment: For cultivation of JCM 19729, supplement with 10.0 g/L sea salts (Sigma). | |||||