483 .
ETHANOLOGENBACTERIUM MEDIUM
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Equipment needed:
Filter
Final pH:
5.5
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Glucose | 10.0 | g | ||
|
|
Tryptone
(BD-Difco) |
4.0 | g | ||
|
|
Beef extract
(BD-Difco) |
2.0 | g | ||
|
|
Yeast extract
(BD-Difco) |
1.0 | g | ||
|
|
NaCl | 4.0 | g | ||
|
|
K2HPO4 | 1.5 | g | ||
|
|
FeSO4 x 7 H2O | 0.1 | g | ||
|
|
MgCl2 x 6 H2O | 0.2 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Microelement solution | 10.0 | ml | ||
|
|
Vitamin solution | 10.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix components except vitamin solution, and adjust pH to 4.5 - 6.5. After autoclaving, aseptically add filter-sterilized vitamin solution. | |||||
Microelement solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
MnSO4 x n H2O | 0.01 | g |
|
|
ZnSO4 x 7 H2O | 0.05 | g |
|
|
H3BO3 | 0.01 | g |
|
|
Nitrilotriacetic acid | 4.50 | g |
|
|
CaCl2 x 2 H2O | 0.01 | g |
|
|
Na2MoO4 x 2 H2O | 0.01 | g |
|
|
CoCl2 x 6 H2O | 0.20 | g |
|
|
AlK(SO4)2 x 12 H2O | 0.01 | g |
|
|
Distilled water | 1000.00 | ml |
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Cyanocobalamin | 0.010 | g |
|
|
Ascorbic acid | 0.025 | g |
|
|
Riboflavin | 0.025 | g |
|
|
Citric acid | 0.020 | g |
|
|
Pyridoxine hydrochloride | 0.050 | g |
|
|
Folic acid | 0.010 | g |
|
|
p-Aminobenzoic acid | 0.010 | g |
|
|
Creatine | 0.025 | g |
|
|
Distilled water | 1000.000 | ml |