Name: MMJ-DO MEDIUM
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. J495 #

Equipment needed: Autoclave Filter

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Modified MJ synthetic seawater	1000.0	ml	-	-
Yeast extract	0.1	g	0.1	-
Sodium pyruvate	0.5	g	0.5	4.544
Sodium lactate	0.5	g	0.5	4.462
Sodium ascorbate	0.1	g	0.1	0.505
NaHCO₃	1.0	g	1	11.904
Resazurin	1.0	mg	1.0e-3	0.004
1 Mix ingredients except NaHCO₃, adjust pH to 7.0 with NaOH and autoclave under a H₂-CO₂ (4:1, v/v) gas mixture. Filter-sterilize 8% NaHCO₃ solution and add to the medium. Pressurize the inoculated bottles to 200 kPa H₂-CO₂ (4:1, v/v).

Modified MJ synthetic seawater #

1000

Solution occurs in 4 other media (first defined in Medium J492).

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
NaCl	30.00	g	30	513.347
K₂HPO₄	0.14	g	0.14	0.804
CaCl₂ x 2 H₂O	0.50	g	0.5	3.401
NH₄Cl	0.50	g	0.5	9.348
MgSO₄ x 7 H₂O	3.40	g	3.4	13.794
MgCl₂ x 6 H₂O	4.18	g	4.18	20.56
KCl	0.33	g	0.33	4.427
FeSO₄ x 7 H₂O	0.01	g	0.01	0.036
NiCl₂ x 6 H₂O	1.00	mg	1.0e-3	0.008
Na₂SeO₃ x 5 H₂O	1.00	mg	1.0e-3	0.004
Trace element solution	10.00	ml	-	-
Distilled water	1000.00	ml	-	-

All strains for this medium:

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