545 .
DESULFOTOMACULUM Lam5 MEDIUM
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Equipment needed:
Autoclave
Filter
Hungate tubes
Final pH:
7.2
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.3 | g | ||
|
|
K2HPO4 | 0.3 | g | ||
|
|
NH4Cl | 1.0 | g | ||
|
|
NaCl | 1.0 | g | ||
|
|
Na2SO4 | 3.0 | g | ||
|
|
KCl | 0.1 | g | ||
|
|
CaCl2 | 0.1 | g | ||
|
|
MgCl2 x 6 H2O | 0.5 | g | ||
|
|
Yeast extract | 0.1 | g | ||
|
|
Sodium lactate | 1.0 | g | ||
|
|
Trace element solution | 10.0 | ml | ||
|
|
NaHCO3 | 2.0 | g | ||
|
|
L-Cysteine HCl x H2O | 0.5 | g | ||
|
|
Na2S x 9 H2O | 0.4 | g | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Mix ingredients except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O and adjust pH to 7.2. Distribute the medium into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a N2 x CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave. After cooling, add solutions of NaHCO3 [5%, filter-sterilized and stored under a N2-CO2 (4:1, v/v) gas mixture], L-cysteine x HCl x H2O and Na2S x 9H2O (5%, autoclaved and stored under N2) to the medium. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%) |
12.5 | ml |
|
|
FeSO4 x 7 H2O | 2.1 | g |
|
|
H3BO3 | 30.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
CuCl2 x 2 H2O | 2.0 | mg |
|
|
ZnSO4 x 7 H2O | 144.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 987.0 | ml |