592 .
MODIFIED MMJS MEDIUM (B)
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Equipment needed:
Autoclave
Filter
Final pH:
6.8
Select a strain (optional):
Solution A #
1026
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 20.00 | g | ||
|
|
K2HPO4 | 0.09 | g | ||
|
|
KH2PO4 | 0.07 | g | ||
|
|
CaCl2 x 2 H2O | 0.80 | g | ||
|
|
NH4Cl | 1.00 | g | ||
|
|
NaNO3 | 1.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
MgCl2 x 6 H2O | 6.00 | g | ||
|
|
KCl | 0.33 | g | ||
|
|
NiCl2 x 6 H2O | 1.00 | mg | ||
|
|
Na2SeO3 x 5 H2O | 1.00 | mg | ||
|
|
Fe2(SO4)3 x n H2O | 0.01 | g | ||
|
|
H2WO4 | 1.00 | mg | ||
|
|
Na2S2O3 x 5 H2O | 3.00 | g | ||
|
|
FeSO4 x 7 H2O | 0.01 | g | ||
|
|
Ferrous citrate | 0.01 | g | ||
|
|
Trace mineral solution | 5.00 | ml | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix ingredients and adjust pH to 6.8 with NaOH. Distribute the solution into culture vessels (leaving more than 80% volume of gas phase in the vessel) under a stream of a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Then, add the following solutions (filter-sterilized) to the medium (per liter): | |||||
|
|
Trace vitamins | 1.00 | ml | ||
|
|
NaHCO3
(5%) |
20.00 | ml | ||
| 2 Finally, replace the gas phase with a N2-CO2-O2 (79:20:1, by volume) gas mixture and pressurize to 200 kPa with the same gas mixture. | |||||
Trace mineral solution #
| 1 Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4 x 2H2O. |
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |