593 .
MODIFIED 9K MEDIUM FOR FERROPLASMA AEOLICUM
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Equipment needed:
Autoclave
Filter
Final pH:
1.6
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 0.4 | g | ||
|
|
(NH4)2SO4 | 0.2 | g | ||
|
|
KCl | 0.1 | g | ||
|
|
K2HPO4 | 0.1 | g | ||
|
|
FeSO4 x 7 H2O | 25.0 | g | ||
|
|
Yeast extract | 0.2 | g | ||
|
|
FeCl2 solution | 1.0 | ml | ||
|
|
Trace element solution | 1.0 | ml | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 1.6 - 1.7 with 10 N H2SO4 and autoclave. After cooling, add filter-sterilized K2S4O6 solution to give a final concentration of 5 mM. | |||||
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |