599 .
MODERATE HALOPHILE MEDIUM
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Equipment needed:
Final pH:
7.1
Select a strain (optional):
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 10.000 | g | ||
|
|
Proteose peptone no. 3
(BD-Difco) |
5.000 | g | ||
|
|
Glucose | 1.000 | g | ||
|
|
NaCl | 81.000 | g | ||
|
|
MgSO4 x 7 H2O | 9.600 | g | ||
|
|
MgCl2 x 6 H2O | 7.000 | g | ||
|
|
CaCl2 x 2 H2O | 0.360 | g | ||
|
|
KCl | 2.000 | g | ||
|
|
NaHCO3 | 0.060 | g | ||
|
|
NaBr | 0.026 | g | ||
| 1 Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2. For preparation of solid medium, add 20.0 g/L agar. | |||||