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JCM 616 . DESULFOMICROBIUM MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J616 #

ml
Equipment needed: Autoclave
1 Mix ingredients and autoclave. Cool under a N2-CO2 gas mixture (4:1, v/v) and add the following solutions from sterile stocks:
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.25 g 0.242 1.778
NH4Cl 0.50 g 0.484 9.049
CaCl2 x 2 H2O 0.05 g 0.048 0.329
MgSO4 x 7 H2O 0.50 g 0.484 1.964
KCl 0.50 g 0.484 6.493
Na2SO4 1.42 g 1.375 9.678
Sodium lactate 1.12 g 1.084 9.675
Trace element solution SL12B 1.00 ml - -
Distilled water 1000.00 ml - -
NaHCO3
(8%) 		30.00 ml - -
Na2S x 9 H2O
(5%) 		1.00 ml - -
Vitamin solution 1.00 ml - -
2 Distribute the medium into culture vessels under the same gas mixture and seal with butyl rubber stoppers. Adjust pH to 7.0 - 7.2, if necessary.

Trace element solution SL12B #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-EDTA 3.0 g 3 -
FeSO4 x 7 H2O 1.1 g 1.1 3.957
CoCl2 x 6 H2O 190.0 mg 0.19 1.463
MnCl4 x 4 H2O 50.0 mg 0.05 -
ZnCl2 42.0 mg 0.042 0.308
NiCl2 x 6 H2O 24.0 mg 0.024 0.185
Na2MoO4 x 2 H2O 18.0 mg 0.018 0.074
H3BO3 300.0 mg 0.3 4.851
CuCl2 x 2 H2O 2.0 mg 0.002 0.012
Distilled water 1000.0 ml - -

Vitamin solution #

1000
Solution occurs in 2 other media (first defined in Medium J616).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 100 mg 0.1 0.074
p-Aminobenzoic acid 80 mg 0.08 0.583
Biotin 20 mg 0.02 0.082
Nicotinic acid 200 mg 0.2 1.625
DL-Calcium pantothenate 100 mg 0.1 0.21
Pyridoxine hydrochloride 300 mg 0.3 -
Thiamine HCl 200 mg 0.2 -
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.1
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Na2SO4 1.375 9.678
Sodium lactate 1.084 9.675
NH4Cl 0.484 9.049
KCl 0.484 6.493
MgSO4 x 7 H2O 0.484 1.964
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Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains