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JCM 647 . THIOCAPSA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J647 #

ml
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
KH2PO4 0.50 g 0.492 3.613
CaCl2 x 2 H2O 0.05 g 0.049 0.334
MgSO4 x 7 H2O 1.00 g 0.983 3.989
NH4Cl 0.64 g 0.629 11.765
NaCl 0.50 g 0.492 8.413
Yeast extract 0.40 g 0.393 -
Sodium pyruvate 3.00 g 2.95 26.807
Micronutrient solution SL7 1.00 ml - -
Ferric citrate
(0.1%, w/v) 		5.00 ml - -
NaHCO3
(10%, w/v) 		10.00 ml - -
Vitamin B12
(2 mg/ml) 		1.00 ml - -
Na2S x 9 H2O 1.00 mM - 1
Na2S2O3 x 5 H2O 6.00 mM - 6
Distilled water 1000.00 ml - -
1 Adjust pH to 7.0.
2 After autoclaving, add filter-sterilized vitamin B12, NaHCO3, Na2S x 9H2O and Na2S2O3 x 5H2O solutions to the medium.

Micronutrient solution SL7 #

1001
Solution occurs in 12 other media (first defined in Medium J533).
Compound Amount Unit Conc. [g/L] Conc. [mM]
HCl
(25%, v/v) 		1 ml - -
ZnCl2 70 mg 0.07 0.513
MnCl2 x 4 H2O 100 mg 0.1 -
H3BO3 60 mg 0.06 0.969
CoCl2 x 6 H2O 200 mg 0.2 1.539
CuCl2 x 2 H2O 20 mg 0.02 0.117
NiCl2 x 6 H2O 20 mg 0.02 0.154
Na2MoO4 x 2 H2O 40 mg 0.04 0.165
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0
Equipment needed: Filter
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sodium pyruvate 2.95 26.807
MgSO4 x 7 H2O 0.983 3.989
NH4Cl 0.629 11.765
NaCl 0.492 8.413
KH2PO4 0.492 3.613
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain