Compound | Amount | Unit | |
---|---|---|---|
|
Marine agar 2216
(BD-Difco) |
55.1 | g |
|
R2A agar
(BD-Difco) |
9.1 | g |
|
Artificial seawater | 750.0 | ml |
|
Distilled water | 250.0 | ml |
Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
NaCl | 24.0 | g | ||
|
MgSO4 x 7 H2O | 7.0 | g | ||
|
MgCl2 x 6 H2O | 5.3 | g | ||
|
KCl | 0.7 | g | ||
|
CaCl2 x 2 H2O | 0.1 | g | ||
|
Distilled water | 1000.0 | ml | ||
1 Adjust pH to 7.5. | |||||
2 Comment: For preparation of the liquid medium, use Marine broth 2216 (BD-Difco) instead of Marine agar 2216 and R2A broth instead of R2A agar by omitting agar from the R2A agar medium composition (see Medium No. 346). |