JCM 659 . PELAGICOCCUS AGAR

What is this page?
Equipment needed:

Final pH: 7.5

Select a strain (optional):

ml
Compound Amount Unit
Marine agar 2216
(BD-Difco)
55.1 g
R2A agar
(BD-Difco)
9.1 g
Artificial seawater 750.0 ml
Distilled water 250.0 ml

Artificial seawater #

1000
Compound Amount Unit
NaCl 24.0 g
MgSO4 x 7 H2O 7.0 g
MgCl2 x 6 H2O 5.3 g
KCl 0.7 g
CaCl2 x 2 H2O 0.1 g
Distilled water 1000.0 ml
1 Adjust pH to 7.5.
2 Comment: For preparation of the liquid medium, use Marine broth 2216 (BD-Difco) instead of Marine agar 2216 and R2A broth instead of R2A agar by omitting agar from the R2A agar medium composition (see Medium No. 346).