698 .
ACIDIMICROBIUM MEDIUM
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Equipment needed:
Autoclave
Final pH:
1.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgSO4 x 7 H2O | 0.5 | g | ||
|
|
(NH4)2SO4 | 0.4 | g | ||
|
|
K2HPO4 | 0.2 | g | ||
|
|
KCl | 0.1 | g | ||
|
|
FeSO4 x 7 H2O | 10.0 | mg | ||
|
|
Distilled water | 1000.0 | ml | ||
| 1 Adjust pH to 2.0 with H2SO4. After autoclaving, add the following solution (autoclaved): | |||||
|
|
Yeast extract
(10%, BD-Difco) |
2.5 | ml | ||
| 2 Comment: For autotrophic growth on ferrous sulfate, prepare the medium without yeast extract, add 13.9 g/L (final) FeSO4 x 7H2O and adjust pH to 1.7 with H2SO4. | |||||