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JCM 699 . MODIFIED THIOCAPSA MEDIUM

Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.

Culture medium recipe

Vol.
×

Main sol. J699 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 1.000 g 1.05 17.974
MgCl2 x 6 H2O 0.400 g 0.42 2.067
KH2PO4 0.200 g 0.21 1.544
KCl 0.500 g 0.525 7.045
NH4Cl 0.125 g 0.131 2.455
CaCl2 x 2 H2O 0.150 g 0.158 1.072
Na2SO4 0.150 g 0.158 1.109
NaNO2 0.069 g 0.072 1.051
Modified trace element solution SL-12 1.000 ml - -
Seven vitamins solution 1.000 ml - -
Distilled water 950.000 ml - -
1 Add NaHCO3 buffer (see below) and adjust pH to 7.2 - 7.4, if necessary.

NaHCO3 buffer #

50
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaHCO3 2.5 g 50 595.188
Distilled water 50.0 ml - -

Modified trace element solution SL-12 #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
H3BO3 300 mg 0.3 4.851
FeSO4 x 7 H2O 1 g 1 3.597
CoCl2 x 6 H2O 190 mg 0.19 1.463
MnCl2 x 4 H2O 100 mg 0.1 -
ZnCl2 70 mg 0.07 0.514
NiCl2 x 6 H2O 24 mg 0.024 0.185
Na2MoO4 x 2 H2O 18 mg 0.018 0.074
CuCl2 x 2 H2O 2 mg 0.002 0.012
Distilled water 1000 ml - -

Seven vitamins solution #

200
Solution occurs in 1 other media (first defined in Medium J699).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 10 mg 0.05 0.037
p-Aminobenzoic acid 10 mg 0.05 0.365
D-(+)-biotin 2 mg 0.01 0.041
Nicotinic acid 20 mg 0.1 0.812
Calcium pantothenate 5 mg 0.025 0.052
Pyridoxine hydrochloride 50 mg 0.25 -
Thiamine-HCl x 2 H2O 10 mg 0.05 -
Distilled water 200 ml - -

Metadata

Last modified: 22.02.22
Source: JCM
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.3
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 1.05 17.974
KCl 0.525 7.045
MgCl2 x 6 H2O 0.42 2.067
KH2PO4 0.21 1.544
Na2SO4 0.158 1.109
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature psychrophilic 1 strain