717 .
TYE MEDIUM FOR STRAIN AeB
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Equipment needed:
Autoclave
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.75 | g | ||
|
|
K2HPO4 | 1.50 | g | ||
|
|
NaCl | 0.90 | g | ||
|
|
MgCl2 x 6 H2O | 0.20 | g | ||
|
|
NH4Cl | 0.90 | g | ||
|
|
Trace mineral solution | 9.00 | ml | ||
|
|
Trace vitamins | 5.00 | ml | ||
|
|
FeSO4 x 7 H2O
(10%) |
5.00 | �l | ||
|
|
Tryptone
(BD-Difco) |
2.00 | g | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
Casamino acids
(BD-Difco) |
2.00 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly. Bring to a boil, cool down and distribute into culture vessels under a N2 gas stream. Seal with butyl rubber stoppers and autoclave. Prior to inoculation, add the following solution (autoclaved under a N2 atmosphere) into the medium (per liter): | |||||
|
|
Na2S x 9 H2O
(5%) |
4.00 | ml | ||
Trace mineral solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 12.50 | g | ||
|
|
FeCl2 x 4 H2O | 0.20 | g | ||
|
|
MnCl2 x 4 H2O | 0.10 | g | ||
|
|
CoCl2 x 6 H2O | 0.02 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
ZnCl2 | 0.10 | g | ||
|
|
CuCl2 | 0.20 | g | ||
|
|
H3BO3 | 0.01 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.01 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
Na2SeO3 x 5 H2O | 0.02 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH. Then proceed to add the minerals. | |||||
Trace vitamins #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |