J724
724 .
RAVOT MODIFIED MEDIUM FOR MARINITOGA LITORALIS
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J724 #
ml
Equipment needed: Autoclave
Anaerobic workbench
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 26.00 | g | 25.515 | 436.605 | |
| MgCl2 x 6 H2O | 0.50 | g | 0.491 | 2.414 | |
| KCl | 0.50 | g | 0.491 | 6.582 | |
| NH4Cl | 0.30 | g | 0.294 | 5.504 | |
| KH2PO4 | 0.10 | g | 0.098 | 0.721 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.098 | 0.668 | |
| Sodium acetate x 3 H2O | 0.83 | g | 0.815 | - | |
| Yeast extract | 2.00 | g | 1.963 | - | |
|
Trypticase peptone
(BD-BBL) |
2.00 | g | 1.963 | - | |
| Maltose | 2.00 | g | 1.963 | 5.734 | |
| PIPES | 3.30 | g | 3.238 | 10.709 | |
|
Sulfur
(powder) |
5.00 | g | 4.907 | 153.002 | |
| Resazurin | 1.00 | mg | 9.8e-4 | 0.004 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Mix components except sulfur, adjust pH to 6.0 and autoclave under a N2 atmosphere. After cooling, add per liter the following solutions (autoclaved and stored anaerobically): | |||||
|
KH2PO4
(7%) |
4.30 | ml | - | - | |
|
K2HPO4
(7%) |
4.30 | ml | - | - | |
| 2 Steam sulfur for 3 hr on each of 3 successive days. Distribute the medium into culture vessels containing sulfur under a N2 atmosphere, and seal with butyl rubber stoppers. Then, add the following solution (autoclaved and stored under a N2 atmosphere) to the medium: | |||||
|
Na2S x 9 H2O
(3%) |
10.00 | ml | - | - | |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Anaerobic workbench, Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 25.515 | 436.605 |
| Sulfur | 4.907 | 153.002 |
| PIPES | 3.238 | 10.709 |
| Maltose | 1.963 | 5.734 |
| Trypticase peptone | 1.963 | - |
| Show more | ||
