Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NaCl | 20.000 | g | 19.066 | 326.245 | |
MgSO4 x 7 H2O | 3.500 | g | 3.337 | 13.537 | |
MgCl2 x 6 H2O | 2.750 | g | 2.622 | 12.895 | |
KCl | 0.325 | g | 0.31 | 4.156 | |
NaBr | 0.050 | g | 0.048 | 0.463 | |
H3BO3 | 0.015 | g | 0.014 | 0.231 | |
SrCl2 x 6 H2O | 7.500 | mg | 0.007 | 0.027 | |
(NH4)2SO4 | 10.000 | mg | 0.01 | 0.072 | |
KI | 0.500 | mg | 4.8e-4 | 0.002 | |
Na2WO2 x 2 H2O | 0.100 | mg | 9.5e-5 | - | |
CaCl2 x 2 H2O | 0.750 | g | 0.715 | 4.863 | |
KH2PO4 | 0.500 | g | 0.477 | 3.503 | |
NiCl2 x 6 H2O | 2.000 | mg | 0.002 | 0.015 | |
Trace minerals (see Medium No. 151 | 10.000 | ml | - | - | |
Resazurin | 1.000 | mg | 9.5e-4 | 0.004 | |
Distilled water | 1000.000 | ml | - | - | |
1 Mix ingredients, bring to a boil and cool under a H2-CO2 (4:1, v/v) gas mixture. Slowly add 10 ml 5% Na2S x 9H2O solution to the medium, wait for the medium to become colorless, then adjust pH to 7.0 with NaOH. Distribute medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Before inoculation, aseptically and anaerobically add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere): | |||||
KNO3
(10%) |
10.000 | ml | - | - | |
PIPES
(pH 7.0, 0.05 M) |
25.000 | ml | - | - | |
Na2S x 9 H2O
(neutralized, 3%) |
4.000 | ml | - | - | |
2 Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v). |
Last modified: | 22.02.22 |
Source: | JCM |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | 7.0 |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 19.066 | 326.245 |
MgSO4 x 7 H2O | 3.337 | 13.537 |
MgCl2 x 6 H2O | 2.622 | 12.895 |
CaCl2 x 2 H2O | 0.715 | 4.863 |
KH2PO4 | 0.477 | 3.503 |
Show more |