784 .
DESULFOSPOROSINUS ACIDOPHILUS MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Hungate tubes
Final pH:
5.8
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 0.450 | g | ||
|
|
KH2PO4 | 0.050 | g | ||
|
|
MgSO4 x 7 H2O | 0.500 | g | ||
|
|
KCl | 0.050 | g | ||
|
|
Yeast extract | 0.500 | g | ||
|
|
Ca(NO3)2 x 4 H2O | 0.014 | g | ||
|
|
Na2SO4 | 3.000 | g | ||
|
|
Trace element solution | 1.000 | ml | ||
|
|
L-Cysteine HCl x H2O | 0.500 | g | ||
|
|
Resazurin | 1.000 | mg | ||
|
|
Distilled water | 900.000 | ml | ||
| 1 Mix components thoroughly, distribute the medium into culture vessels (e.g., 4.5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (per 900 ml medium) from anaerobic stocks (autoclaved or *filter-sterilized): | |||||
|
|
Na2HPO4
(5%) |
20.000 | ml | ||
|
|
KH2PO4
(25%) |
40.000 | ml | ||
|
|
Fructose
(1 M) |
20.000 | ml | ||
|
|
Trace vitamins (see Medium No. 197)
(*) |
20.000 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
8.000 | ml | ||
| 2 Check final pH of the medium to be around 5.8. | |||||
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(25%) |
12.5 | ml |
|
|
FeSO4 x 7 H2O | 2.1 | g |
|
|
H3BO3 | 30.0 | mg |
|
|
MnCl2 x 4 H2O | 100.0 | mg |
|
|
CoCl2 x 6 H2O | 190.0 | mg |
|
|
NiCl2 x 6 H2O | 24.0 | mg |
|
|
CuCl2 x 2 H2O | 2.0 | mg |
|
|
ZnSO4 x 7 H2O | 144.0 | mg |
|
|
Na2MoO4 x 2 H2O | 36.0 | mg |
|
|
Distilled water | 987.0 | ml |