824 .
DESULFOHALOBIUM MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Final pH:
7.0
Select a strain (optional):
Solution A #
902
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NH4Cl | 1.0 | g | ||
|
|
KH2PO4 | 0.3 | g | ||
|
|
K2HPO4 | 0.3 | g | ||
|
|
NaCl | 100.0 | g | ||
|
|
KCl | 4.0 | g | ||
|
|
Na2SO4 | 3.0 | g | ||
|
|
Sodium acetate | 1.0 | g | ||
|
|
Sodium lactate | 1.0 | g | ||
|
|
Yeast extract | 1.0 | g | ||
|
|
Trypticase peptone
(BD-BBL) |
1.0 | g | ||
|
|
FeCl2 solution | 1.0 | ml | ||
|
|
Trace element solution | 1.0 | ml | ||
|
|
Na2SeO3 x 5 H2O | 3.0 | �g | ||
|
|
Resazurin | 1.0 | mg | ||
|
|
Distilled water | 900.0 | ml | ||
| 1 Mix components thoroughly and adjust pH to 7.0. Autoclave under a N2 atmosphere. | |||||
Solution B #
110
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
MgCl2 x 6 H2O | 20.0 | g | ||
|
|
CaCl2 x 2 H2O | 2.7 | g | ||
|
|
Distilled water | 100.0 | ml | ||
| 1 To complete the medium, combine Solutions A and B under a N2 atmosphere. Then, add per liter the following solution from an anaerobic stock: | |||||
|
|
Na2S x 9 H2O
(3%) |
10.0 | ml | ||
| 2 Autoclave under a N2 atmosphere. | |||||
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |