JCM 88 . 1% OGAWA MEDIUM

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Equipment needed: Filter

Final pH: n.d.

Select a strain (optional):

Solution I #

1000
Compound Amount Unit
Na glutamate 10 g
KH2PO4 10 g
Distilled water 1000 ml

Solution II #

212
Compound Amount Unit
Glycerol 6 ml
Malachite green
(2%)
6 ml
Whole egg 200 ml
1 Homogenize Solution II by stirring and filter through gauze. Mix solutions I and II, and sterilize at 90C for 60 min.
2 Comment: Commercial 1% Ogawa medium (Eiken or Nissui) is also available.