J893
893 .
MBBS MEDIUM FOR HYDROGENOBACTER
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J893 #
ml
Equipment needed: Filter
Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Modified Brock's salt base solution | 1000 | ml | - | - | |
|
Yeast extract
(10%) |
5 | ml | - | - | |
|
NaHCO3
(filter-sterilized, 8%) |
10 | ml | - | - | |
|
Sulfur
(powder) |
1 | g | 0.985 | 30.721 | |
| 1 Autoclave the base solution and the yeast extract solution separately. Filter-sterilize the NaHCO3 solution and steam sulfur for 3 hr on each of 3 successive 3 days. Aseptically mix the three solutions and distribute into culture vessels containing sulfur under a H2-CO2-O2 (80:20:2, v/v) gas stream and seal with butyl rubber stoppers. Adjust pH to 6.0 with sterile 1 N H2SO4, if necessary. Pressurize inoculated bottles to 100 kPa with the same gas mixture. | |||||
Solution occurs in 13
other media (first defined in Medium J165).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| (NH4)2SO4 | 1.30 | g | 1.3 | 9.838 |
| KH2PO4 | 0.28 | g | 0.28 | 2.058 |
| MgSO4 x 7 H2O | 0.25 | g | 0.25 | 1.014 |
| CaCl2 x 2 H2O | 0.07 | g | 0.07 | 0.476 |
| FeCl3 x 6 H2O | 2.00 | mg | 0.002 | 0.007 |
| MnCl2 x 4 H2O | 1.80 | mg | 0.002 | 0.009 |
| Na2B4O7 x 10 H2O | 4.50 | mg | 0.005 | - |
| ZnSO4 x 7 H2O | 0.22 | mg | 2.2e-4 | 7.7e-4 |
| CuCl2 x 2 H2O | 0.05 | mg | 5.0e-5 | 2.9e-4 |
| Na2MoO4 x 2 H2O | 0.03 | mg | 3.0e-5 | 1.2e-4 |
| VOSO4 x n H2O | 0.03 | mg | 3.0e-5 | - |
| CoSO4 x 7 H2O | 0.01 | mg | 1.0e-5 | 6.5e-5 |
| Distilled water | 1000.00 | ml | - | - |
Metadata
| Last modified: | 22.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| (NH4)2SO4 | 1.281 | 9.693 |
| Sulfur | 0.985 | 30.721 |
| KH2PO4 | 0.276 | 2.027 |
| MgSO4 x 7 H2O | 0.246 | 0.999 |
| CaCl2 x 2 H2O | 0.069 | 0.469 |
| Show more | ||
