899 .
THERMOBACTER ROSEUM MEDIUM
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Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.20 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
MgCl2 x 6 H2O | 0.40 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
NaHCO3 | 0.10 | g | ||
|
|
Resazurin | 1.00 | mg | ||
|
|
Yeast extract
(BD-Difco) |
2.00 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly and autoclave under a N2 gas atmosphere. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): | |||||
|
|
Vitamin solution
(*) |
1.00 | ml | ||
|
|
Trace element solution
(*) |
1.00 | ml | ||
|
|
Na2S x 9 H2O
(5%) |
6.00 | ml | ||
| 2 Check pH to be 7.5 - 7.6. | |||||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
p-Aminobenzoic acid | 25.0 | mg |
|
|
Biotin | 100.0 | mg |
|
|
Nicotinic acid | 25.0 | mg |
|
|
DL-Calcium pantothenate | 25.0 | mg |
|
|
Pyridoxine hydrochloride | 25.0 | mg |
|
|
Folic acid | 10.0 | mg |
|
|
Riboflavin | 25.0 | mg |
|
|
Vitamin B12 | 0.5 | mg |
|
|
Lipoic acid | 25.0 | mg |
|
|
Distilled water | 1000.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Fe(NH4)2(SO4)2 x 6 H2O | 784 | mg |
|
|
CoCl2 x 6 H2O | 238 | mg |
|
|
(NH4)2Ni(SO4)2 x 6 H2O | 395 | mg |
|
|
Na2MoO4 x 2 H2O | 24 | mg |
|
|
Na2WO4 x 2 H2O | 33 | mg |
|
|
ZnSO4 x 7 H2O | 144 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
Na2SeO4 | 94 | mg |
|
|
H3BO3 | 6 | mg |
|
|
MnCl2 x 4 H2O | 99 | mg |
|
|
Distilled water | 1000 | ml |