940 .
AMINIVIBRIO ASINOENSIS MEDIUM
What is this page?
Equipment needed:
Anaerobic workbench
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
KH2PO4 | 0.20 | g | ||
|
|
NH4Cl | 0.25 | g | ||
|
|
NaCl | 1.00 | g | ||
|
|
KCl | 0.50 | g | ||
|
|
MgCl2 x 6 H2O | 0.40 | g | ||
|
|
CaCl2 x 2 H2O | 0.15 | g | ||
|
|
Sodium pyruvate | 1.10 | g | ||
|
|
Yeast extract | 2.00 | g | ||
|
|
FeCl2 solution | 1.00 | ml | ||
|
|
Trace element solution | 1.00 | ml | ||
|
|
Selenite-tungstate solution | 1.00 | ml | ||
|
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Resazurin | 0.40 | mg | ||
|
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Distilled water | 1000.00 | ml | ||
| 1 Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (filter-sterilized): | |||||
|
|
NaHCO3
(8%) |
30.00 | ml | ||
|
|
Vitamin solution | 1.00 | ml | ||
| 2 Distribute the medium into culture vessels under the same gas mixture. Finally, add per liter the following solution from an anaerobic stock: | |||||
|
|
Na2S x 9 H2O
(5%) |
7.00 | ml | ||
Vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
p-Aminobenzoic acid | 80 | mg |
|
|
Biotin | 20 | mg |
|
|
Thiamine HCl | 200 | mg |
|
|
Vitamin B12 | 50 | mg |
|
|
Distilled water | 1000 | ml |
FeCl2 solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
HCl
(7.7 M, 25%) |
10.0 | ml |
|
|
FeCl2 x 4 H2O | 1.5 | g |
|
|
Distilled water | 990.0 | ml |
Trace element solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
ZnCl2 | 70 | mg |
|
|
MnCl2 x 4 H2O | 100 | mg |
|
|
H3BO3 | 6 | mg |
|
|
CoCl2 x 6 H2O | 190 | mg |
|
|
CuCl2 x 2 H2O | 2 | mg |
|
|
NiCl2 x 6 H2O | 24 | mg |
|
|
Na2MoO4 x 2 H2O | 36 | mg |
|
|
Distilled water | 1000 | ml |
Selenite-tungstate solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
NaOH | 0.4 | g |
|
|
Na2SeO3 x 5 H2O | 6.0 | mg |
|
|
Na2WO4 x 2 H2O | 8.0 | mg |
|
|
Distilled water | 1000.0 | ml |