J946
946 .
KORTHOF'S MEDIUM
Inofficial medium: This medium was provided by external sources and is possibly not curated by experts.
Culture medium recipe
Vol.
×
Main sol. J946 #
ml
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| 10 x Korthof's basal medium | 50 | ml | - | - | |
|
Rabbit serum
(heat-inactivated at 56℃ for 30 min) |
50 | ml | - | - | |
| Distilled water | 400 | ml | - | - | |
| 1 Autoclave distilled water. After autoclaving, allow to cool, aseptically add 10 x Korthof's basal medium and heat-inactivated rabbit serum that has been filter-sterilized ahead of time. | |||||
Equipment needed: Autoclave
Filter
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 7.0 | g | 15.556 | 266.18 | |
| Na2HPO4 | 4.4 | g | 9.778 | 68.878 | |
|
Polypeptone
(BD-BBL) |
4.0 | g | 8.889 | - | |
| KH2PO4 | 1.2 | g | 2.667 | 19.596 | |
| NaHCO3 | 0.1 | g | 0.222 | 2.645 | |
| KCl | 0.2 | g | 0.444 | 5.962 | |
| CaCl2 x 2 H2O | 0.2 | g | 0.444 | 3.023 | |
| Distilled water | 450.0 | ml | - | - | |
| 1 Mix ingredients except CaCl2 and autoclave. Dissolve CaCl2 in 50 ml distilled water and filter-sterilize. After autoclaving, allow to cool, add sterile CaCl2 solution to the medium. | |||||
Metadata
| Last modified: | 24.02.22 |
| Source: | JCM |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | n.d. |
| Equipment needed: | Autoclave, Filter |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 5 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 1.556 | 26.618 |
| Na2HPO4 | 0.978 | 6.888 |
| Polypeptone | 0.889 | - |
| KH2PO4 | 0.267 | 1.96 |
| KCl | 0.044 | 0.596 |
| Show more | ||
