975 .
SALINIVIBRIO SHARMENSIS MEDIUM
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Equipment needed:
Autoclave
Final pH:
9.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
NaCl | 100.00 | g | ||
|
|
Trisodium citrate | 3.00 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
KCl | 2.00 | g | ||
|
|
MnCl2 x 4 H2O | 0.36 | mg | ||
|
|
FeSO4 x 7 H2O | 0.05 | g | ||
|
|
Yeast extract
(BD-Difco) |
10.00 | g | ||
| 1 Add components to distilled water and bring volume to 970 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add the following solution (separately autoclaved): | |||||
|
|
Na2CO3
(w/v, 10%) |
30.00 | ml | ||
| 2 Check the final pH to be around 9.0. | |||||