Name: N27 RHODOSPIRILLACEAE MEDIUM (modified)
Creator: MediaDive
License: http://creativecommons.org/licenses/by/4.0/

Vol.

Main sol. 27 #

Equipment needed: Tungsten lamp Autoclave

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
Yeast extract	0.30	g	0.3	-
Disodium succinate	1.00	g	1	6.171
Ammonium acetate	0.50	g	0.5	6.487
Fe(III) citrate (0.1% in H₂O)	5.00	ml	0.005	0.02
KH₂PO₄	0.50	g	0.5	3.674
MgSO₄ x 7 H₂O	0.40	g	0.4	1.623
NaCl	0.40	g	0.4	6.845
NH₄Cl	0.40	g	0.4	7.478
CaCl₂ x 2 H₂O	0.05	g	0.05	0.34
Vitamin B₁₂ (10 mg in 100 ml H₂O)	0.40	ml	-	-
Trace element solution SL-6	1.00	ml	-	-
Distilled water	1000.00	ml	-	-
Ethanol	0.50	ml	-	-
1 Adjust pH to 6.8.
2 Autoclave at 121°C for 15 min.

Trace element solution SL-6 #

1000

Compound	Amount	Unit	Conc. [g/L]	Conc. [mM]
ZnSO₄ x 7 H₂O	0.10	g	0.1	0.348
MnCl₂ x 4 H₂O	0.03	g	0.03	0.152
H₃BO₃	0.30	g	0.3	4.851
CoCl₂ x 6 H₂O	0.20	g	0.2	1.54
CuCl₂ x 2 H₂O	0.01	g	0.01	0.059
NiCl₂ x 6 H₂O	0.02	g	0.02	0.154
Na₂MoO₄ x 2 H₂O	0.03	g	0.03	0.124
Distilled water	1000.00	ml	-	-