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Main sol. 1526c

Internal Solution-ID: S1104

Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 60.0 g 58.824 1006.52
2 6.0 g 5.882 23.866
3 1.5 g 1.471 19.726
4 1.0 g 0.98 3.95
5 1.0 g 0.98 18.328
6 0.4 g 0.392 2.667
7 0.4 g 0.392 2.252
8 Trace element solution 10.0 ml - -
9
(BD Bacto)
1.0 g 0.98 -
10
(0.1% w/v)
0.5 ml 4.9e-4 0.002
11 1.5 g 1.471 13.875
12 2.5 g 2.451 22.273
13 Vitamin solution 10.0 ml - -
14 0.5 g 0.49 2.041
15 1000.0 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients except carbonate, pyruvate, vitamins, and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add pyruvate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas ( pyruvate and vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Prior to use adjust pH of complete medium to 7.3 - 7.5, if necessary.
  2. Note: Use at least 5-10%(v/v) inoculum.
1526c DESULFOHALOVIBRIO (L21 LS) MEDIUM