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505. FORMIVIBRIO MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Solution A 940.00 ml - -
2 Solution B 1.00 ml - -
3 Solution C 1.00 ml - -
4 Solution D 10.00 ml - -
5 Solution E 30.00 ml - -
6 Solution F 10.00 ml - -
7 Solution G 10.00 ml - -
9 , might need strain adjustment 10.00 mM - -
10 , might need strain adjustment 0.20 % - -
11 , might need strain adjustment 0.20 % - -
12 , might need strain adjustment 0.05 g/l - -
Instructions:
  1. Sparge solution A with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Solutions B, D and G are autoclaved separately under 100% N2 gas. Solutions C and F are prepared under 100% N2 gas and sterilized by filtration. Solution E is autoclaved und 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to G are added to the sterile solution A in the sequence as indicated. Adjust pH of complete medium to 7.5 - 7.8 with a sterile anoxic stock solution of Na2CO3, if necessary.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.40 g 1.489 10.944
2 0.50 g 0.532 9.944
3 0.20 g 0.213 1.047
4 0.15 g 0.16 1.085
5
(0.1% w/v)
0.50 ml 5.3e-4 0.002
6 940.00 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Trace element solution SL-10 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Selenite-tungstate solution 1 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Vitamin solution 10 ml - -
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.5 g 50 471.752
2 30.0 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 3 g 300 1020.06
2 10 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.3 g 30 124.905
2 10.0 ml 998.207 55408.8
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8
Solution occurs in 239 other media (first defined in medium 320 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
2
(25%; 7.7 M)
10.0 ml 3.17 86.943
3 1.5 g 1.5 7.545
4 70.0 mg 0.07 0.514
5 100.0 mg 0.1 0.505
6 6.0 mg 0.006 0.097
7 190.0 mg 0.19 0.799
8 2.0 mg 0.002 0.012
9 24.0 mg 0.024 0.101
10 36.0 mg 0.036 0.149
11 990.0 ml 988.225 54854.8
Instructions:
  1. First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.0 ml.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.8
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 2
Strains with modifications: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.197 55408.2
Na3-citrate x 2 H2O 2.994 10.18
Na2CO3 1.497 14.124
KH2PO4 1.397 10.267
NH4Cl 0.499 9.329
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Cultivation metadata from 2 strains BacDive

phyla 2 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains

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