Solution A
Internal Solution-ID: S1253
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Yeast extract
(Difco 0127) |
10 | g | 20.408 | - | |
|
ACES
(N-2-acetamido-2-aminoethane-sulfonic acid) |
10 | g | 20.408 | 112.01 | |
|
Activated charcoal
(Sigma C 5510) |
2 | g | 4.082 | - | |
| Distilled water | 490 | ml | - | - | |
| 1 Dissolve ACES and bring to pH 6.9 by slowly adding 1 N KOH. Add the other components and dissolve by boiling. Autoclave at 121°C for 15 minutes. Cool to 50 - 55°C. | |||||
Media with this solution
| 585 | BCYE AGAR |