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Main sol. 590

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Internal Solution-ID: S1266

Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(Difco, Bacto ) 		1.0 g 1 -
Na2-DL-malate 1.0 g 1 5.616
Hutner's salts 20.0 ml - -
Artificial sea water, 3 times concentrated 980.0 ml - -
Agar , for solid medium 18.0 g/l 18 53.519
NaHCO3
(8 %) 		28.4 ml 2.272 27.045
1 pH 7.0-7.2
2 Agar at a concentration of 18 g/l is added for solid media.
3 Prepare an 8% solution of NaHCO3 in half full 100ml serum bottles closed with a rubber septum and autoclave. Add 28.4 ml of the sterile 8% solution of NaHCO3 (2.822g/l) per litre of the sterile, cooled medium.
590 DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM