DSMZ 590 . DICHOTOMICROBIUM THERMOHALOPHILUM MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract
(Difco, Bacto )
1.0 g 1 -
Na2-DL-malate 1.0 g 1 5.616
Hutner's salts 20.0 ml - -
Artificial sea water, 3 times concentrated 980.0 ml - -
Agar , for solid medium 18.0 g/l 18 53.519
NaHCO3
(8 %)
28.4 ml 2.272 27.045
1 pH 7.0-7.2
2 Agar at a concentration of 18 g/l is added for solid media.
3 Prepare an 8% solution of NaHCO3 in half full 100ml serum bottles closed with a rubber septum and autoclave. Add 28.4 ml of the sterile 8% solution of NaHCO3 (2.822g/l) per litre of the sterile, cooled medium.
Solution occurs in 1 other media (first defined in Medium 590).
Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 70.43 g 70.43 1205.17
Na2SO4 11.75 g 11.75 82.723
NaHCO3 2.88 g 2.88 34.283
KCl 1.99 g 1.99 26.694
KBr 0.29 g 0.29 2.437
MgCl2 x 6 H2O 31.86 g 31.86 156.712
CaCl2 x 2 H2O 4.35 g 4.35 29.589
H3BO3 0.08 g 0.08 1.294
Distilled water 1000.00 ml - -
1 Add NaHCO3 from a filter-sterilized stock solution when the medium has cooled.
Solution occurs in 24 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid
(NTA)
10.000 g 10 52.318
MgSO4 x 7 H2O 29.700 g 29.7 120.499
CaCl2 x 2 H2O 3.335 g 3.335 22.685
(NH4)6Mo7O24 x 4 H2O 9.250 mg 0.009 -
FeSO4 x 7 H2O 99.000 mg 0.099 0.356
Metall salt sol. 44 50.000 ml - -
Distilled water 950.000 ml - -
1 Dissolve the nitrilotriacetic acid, adjust the pH to 7.0 with KOH (about 7.3 g). Dissolve other salts separately, combine and adjust the pH to 6.8 with NaOH or H2SO4.
Solution occurs in 18 other media (first defined in Medium 590).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na-EDTA 250.0 mg 0.25 0.744
ZnSO4 x 7 H2O 1095.0 mg 1.095 3.808
FeSO4 x 7 H2O 500.0 mg 0.5 1.798
MnSO4 x H2O 154.0 mg 0.154 0.911
CuSO4 x 5 H2O 39.2 mg 0.039 0.157
Co(NO3)2 x 6 H2O 24.8 mg 0.025 0.085
Na2B4O7 x 10 H2O 17.7 mg 0.018 -
Distilled water 1000.0 ml - -
1 Dissolve the EDTA and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metal ions.

Metadata

Last modified: 24.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.0 - 7.2
Equipment needed: Filter
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Associated strains

All strains for this medium: 4

Molecular composition

Compound Conc. [g/L] Conc. [mM]
NaCl 69.021 1181.07
MgCl2 x 6 H2O 31.223 153.578
Agar 18 53.519
Na2SO4 11.515 81.069
CaCl2 x 2 H2O 4.263 28.997
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Cultivation metadata from 4 strains BacDive

phyla 1 4 strains
temperature thermophilic 4 strains