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Vitamin solution

Internal Solution-ID: S1285

This solution is used in 3 media and was first defined in medium 600a.

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.2 mg 2.0e-4 1.5e-4
Biotin 4.0 mg 0.004 0.016
Thiamine-HCl x 2 H2O 10.0 mg 0.01 0.027
Calcium pantothenate 10.0 mg 0.01 0.021
Folic acid 1.0 mg 1.0e-3 0.002
Riboflavin 10.0 mg 0.01 0.027
Nicotinamide 10.0 mg 0.01 0.082
p-Aminobenzoic acid 10.0 mg 0.01 0.073
Pyridoxine hydrochloride 10.0 mg 0.01 0.049
Distilled water 1000.0 ml - -
1 Filter-sterilized.
600b M13 (3x) PIRELLULA MEDIUM
600a M13a (PIRELLULA) MEDIUM
1586 ISP2 MODIFIED MEDIUM FOR METHYLIBIUM