Main sol. 602
Internal Solution-ID: S1292
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 80.0 | g | 80 | 1368.93 | |
| MgSO4 x 7 H2O | 9.5 | g | 9.5 | 38.543 | |
| KCl | 0.5 | g | 0.5 | 6.707 | |
| CaCl2 x 2 H2O | 0.2 | g | 0.2 | 1.36 | |
| (NH4)2SO4 | 0.1 | g | 0.1 | 0.757 | |
| KNO3 | 0.1 | g | 0.1 | 0.989 | |
| Yeast extract | 1.0 | g | 1 | - | |
| Distilled water | 960.0 | ml | - | - | |
| Hutner's salts | 20.0 | ml | - | - | |
| Vitamin solution | 1.0 | ml | - | - | |
| Phosphate supplement | 20.0 | ml | - | - | |
| 1 pH 8.0 | |||||
| 2 Autoclave and add, on cooling 20.0 ml sterile Hutner's salts (see medium 590), 1.0 ml vitamin solution, and 20.0 ml sterile phosphate supplement. | |||||
Media with this solution
| 602 | HALOMONAS SUBGLACIESCOLA |