Main sol. 634a
Internal Solution-ID: S1353
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| dehydrated RCM medium | 38.0 | g | 38 | - | |
| Casamino acids | 15.0 | g | 15 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) and Casamino acids (15.0 g/l) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense the medium into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.8. | |||||
Media with this solution
| 634a | RCM MEDIUM (with Casamino acids) |