DSMZ 634a . RCM MEDIUM (with Casamino acids)

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
dehydrated RCM medium 38.0 g 38 -
Casamino acids 15.0 g 15 -
Sodium resazurin
(0.1% w/v)
0.5 ml 5.0e-4 0.002
Distilled water 1000.0 ml - -
1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) and Casamino acids (15.0 g/l) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 100% N2 gas. Dispense the medium into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.8.


Last modified: 11.10.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % N2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
dehydrated RCM medium 38 -
Casamino acids 15 -
Sodium resazurin 5.0e-4 0.002
Distilled water - -

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen anaerobe 2 strains
temperature mesophilic 2 strains