Main sol. 634c
Internal Solution-ID: S1355
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
dehydrated RCM medium | 38.0 | g | 38 | - | |
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | 0.002 | |
Sodium carbonate | 1.0 | g | 1 | - | |
Distilled water | 1000.0 | ml | - | - | |
1 Dissolve dehydrated RCM medium (available from BD BBL, OXOID or MERCK) in distilled water and add 0.5 ml/l Na-resazurin solution (0.1% w/v), bring to the boil and cool to room temperature while sparging with 80% N2 and 20% CO2 gas mixture. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Before use, add 1.0 g of sodium carbonate per liter medium from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Check pH of medium after autoclaving and adjust pH to 6.8, if necessary. |
634c | RCM MEDIUM (N2/CO2) |