Main sol. 635
Internal Solution-ID: S1356
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| D-Glucose | 2.00 | g | 1.988 | 11.035 | |
|
Tryptone
(BD BACTO) |
2.00 | g | 1.988 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| L-Cysteine HCl x H2O | 0.25 | g | 0.249 | 1.415 | |
| Trace element solution SL-10 | 10.00 | ml | - | - | |
| Modified Wolin's mineral solution | 25.00 | ml | - | - | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Distilled water | 970.00 | ml | - | - | |
| 1 Dissolve ingredients (except cysteine, trace elements and vitamins), sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Then add cysteine, adjust pH to 7.0, dispense medium under same gas atmosphere in anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add trace elements and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas. Vitamins should be sterilized by filtration. Check pH of complete medium and adjust to 7.0, if necessary. | |||||
Media with this solution
| 635 | GRACILINEMA MEDIUM |