Main sol. 644
Internal Solution-ID: S1368
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NaCl | 15.00 | g | 15 | 256.674 | |
| K2HPO4 | 1.00 | g | 1 | 5.741 | |
| KH2PO4 | 0.15 | g | 0.15 | 1.102 | |
| NH4Cl | 0.50 | g | 0.5 | 9.348 | |
| Na-acetate | 0.82 | g | 0.82 | 9.996 | |
| Trace elements solution (Pfennig & Lippert,1966) | 1.00 | ml | - | - | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
| Yeast extract | 0.20 | g | 0.2 | - | |
| D-Glucose | 1.00 | g | 1 | 5.551 | |
| Agar , for solid medium | 15.00 | g | 15 | 44.599 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve sodium chloride, hydrogenphosphates, ammonium chloride and acetate, adjust pH of solution to 7.0 - 7.2 and autoclave. After autoclaving add trace elements, magnesium sulfate, calcium chloride, yeast extract and glucose from sterile stock solutions. Adjust pH of complete medium to 7.0, if necessary. | |||||
Media with this solution
| 644 | CATENOCOCCUS MEDIUM |