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Main sol. 645

Internal Solution-ID: S1369

This solution is used in 2 mediaand was first defined in medium 645.

Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto Middlebrook 7H10 agar
(Difco 262710)
19 g 20.995 -
Glycerol 5 ml - -
Distilled water 900 ml - -
1 Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.
2 If you can´t order the commercial media then prepare the media in this way:
645 MIDDLEBROOK MEDIUM
780 MIDDLEBROOK MEDIUM WITH MYCOBACTIN