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Culture medium recipe

Compound Amount Unit Conc. [g/L] Conc. [mM]
Mycobactine J 2 mg 0.002 -
Absolute ethanol 4 ml - -
Medium 645 1000 ml - -
1 Reconstitute the contents of one vial of 2 mg Mycobactine J with 2 ml absolute ethanol (analytical grade). Shake carefully so that the product is completely dissolved. Pour the contents of the bottle into 1 litre of medium 645 kept melted in a water bath at +56°C, taking care to pour out all of the product. Rinse out the vial that contained the Mycobactine J with an additional 2 ml of absolute ethanol and pour into the culture medium. Mix the Mycobactine J alcohol solution and the culture medium very thoroughly.
Solution occurs in 1 other media (first defined in Medium 645).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto Middlebrook 7H10 agar
(Difco 262710)
19 g 20.995 -
Glycerol 5 ml - -
Distilled water 900 ml - -
1 Sterilize for 15 min by 121°C cool to 50 - 55°C. Add 100 ml Middlebrook OADC enrichment (Becton Dickinson 212240) under aseptic conditions and mix thoroughly.
2 If you can´t order the commercial media then prepare the media in this way:


Last modified: 24.11.21
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
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Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Bacto Middlebrook 7H10 agar 20.995 -
Mycobactine J 0.002 -
Absolute ethanol - -
Glycerol - -
Distilled water - -

Cultivation metadata from 2 strains BacDive

phyla 1 2 strains
oxygen microaerophile 1 strain
temperature mesophilic 2 strains