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Main sol. 659

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Internal Solution-ID: S1390

Equipment needed: Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Casein peptone 5.00 g 5 -
Soy peptone 5.00 g 5 -
Meat peptone 5.00 g 5 -
Yeast extract 2.50 g 2.5 -
Ascorbic acid 0.50 g 0.5 2.839
MgSO4 x 7 H2O 0.25 g 0.25 1.014
Na2-ß-glycerolphosphate 9.50 g 9.5 43.974
Distilled water 1000.00 ml - -
1 After autoclaving add a lactose solution (sterilized by filtration) up to a concentration of 8.0 g/l, and 1.2 ml 1M CaCl2 x 2 H2O. Adjust pH to 7.15 ± 0.05.
Lactose
(sterilized by filtration) 		8.00 g/l 8 23.371
CaCl2 x 2 H2O 1.20 ml - -
659 M17 MEDIUM (modified)