Main sol. 696
Internal Solution-ID: S1447
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Peptone | 10.00 | g | 10 | - | |
| Meat extract | 8.00 | g | 8 | - | |
| Yeast extract | 3.00 | g | 3 | - | |
| KH2PO4 | 2.50 | g | 2.5 | 18.371 | |
| K2HPO4 | 2.50 | g | 2.5 | 14.353 | |
| MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
| MnSO4 x 4 H2O | 0.05 | g | 0.05 | 0.224 | |
| Glucose | 5.00 | g | 5 | 27.753 | |
| Lactose | 5.00 | g | 5 | 14.607 | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 pH 6.8 | |||||
| 2 After sterilization add 5% bovine serum. | |||||
| Bovine serum | 5.00 | % | 50 | - | |
| 3 For anaerobic handling prepare and distribute into tubes under 100% N2 by using the Hungate technique. | |||||
Media with this solution
| 696 | STREPTOCOCCUS SUIS MEDIUM |