DSMZ 696 . STREPTOCOCCUS SUIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 10.00 g 10 -
Meat extract 8.00 g 8 -
Yeast extract 3.00 g 3 -
KH2PO4 2.50 g 2.5 18.371
K2HPO4 2.50 g 2.5 14.353
MgSO4 x 7 H2O 0.20 g 0.2 0.811
MnSO4 x 4 H2O 0.05 g 0.05 0.224
Glucose 5.00 g 5 27.753
Lactose 5.00 g 5 14.607
L-Cysteine HCl x H2O 0.50 g 0.5 2.847
Distilled water 1000.00 ml - -
1 pH 6.8
2 After sterilization add 5% bovine serum.
Bovine serum 5.00 % 50 -
3 For anaerobic handling prepare and distribute into tubes under 100% N2 by using the Hungate technique.

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.8
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Bovine serum 50 -
Peptone 10 -
Meat extract 8 -
Glucose 5 27.753
Lactose 5 14.607
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen aerobe 1 strain
oxygen anaerobe 1 strain
oxygen facultative anaerobe 1 strain
oxygen microaerophile 1 strain
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