Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Peptone | 10.00 | g | 10 | - | |
Meat extract | 8.00 | g | 8 | - | |
Yeast extract | 3.00 | g | 3 | - | |
KH2PO4 | 2.50 | g | 2.5 | 18.371 | |
K2HPO4 | 2.50 | g | 2.5 | 14.353 | |
MgSO4 x 7 H2O | 0.20 | g | 0.2 | 0.811 | |
MnSO4 x 4 H2O | 0.05 | g | 0.05 | 0.224 | |
Glucose | 5.00 | g | 5 | 27.753 | |
Lactose | 5.00 | g | 5 | 14.607 | |
L-Cysteine HCl x H2O | 0.50 | g | 0.5 | 2.847 | |
Distilled water | 1000.00 | ml | - | - | |
1 pH 6.8 | |||||
2 After sterilization add 5% bovine serum. | |||||
Bovine serum | 5.00 | % | 50 | - | |
3 For anaerobic handling prepare and distribute into tubes under 100% N2 by using the Hungate technique. |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.8 |
Equipment needed: | Autoclave, Gassing station, Hungate tubes |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Bovine serum | 50 | - |
Peptone | 10 | - |
Meat extract | 8 | - |
Glucose | 5 | 27.753 |
Lactose | 5 | 14.607 |
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