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Main sol. 700

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Internal Solution-ID: S1451

Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2CO3 10.0 g 10 94.35
NaHCO3 15.0 g 15 178.557
NaCl 10.0 g 10 171.116
K2HPO4 0.2 g 0.2 1.148
NH4Cl 1.0 g 1 18.695
KCl 0.2 g 0.2 2.683
Na2S x 9 H2O 1.0 g 1 4.164
Yeast extract 0.5 g 0.5 -
Sucrose 5.0 g 5 14.607
Wolin's vitamin solution 10.0 ml - -
Modified Wolin's mineral solution II 1.0 ml - -
Distilled water 1000.0 ml - -
1 Prepare the medium under N2, omitting Na2CO3, Na HCO3, Na2S, sucrose and vitamin solution.
2 Boil the medium and cool under nitrogen, add the Na2CO3 and NaHCO3 and adjust the pH to 9.7 with 6N NaOH (about 15 ml). Sterilize by autoclaving and add the Na2S (neutralized), vitamins and sucrose from sterile stock solutions.
700 ALKALIPHILIC SPIROCHAETE MEDIUM