Main sol. 720
Internal Solution-ID: S1472
Equipment needed: Filter
Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| NH4Cl | 1.00 | g | 0.997 | 18.639 | |
| NaCl | 0.10 | g | 0.1 | 1.706 | |
| MgCl2 x 6 H2O | 0.10 | g | 0.1 | 0.49 | |
| CaCl2 x 2 H2O | 0.05 | g | 0.05 | 0.339 | |
| K2HPO4 x 3 H2O | 0.40 | g | 0.399 | 2.29 | |
| Trace element solution SL-11 | 1.00 | ml | - | - | |
| Selenite-tungstate solution | 1.00 | ml | - | - | |
| Yeast extract | 1.00 | g | 0.997 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| NaHCO3 | 2.60 | g | 2.592 | 30.857 | |
| Na-pyruvate | 2.50 | g | 2.493 | 22.65 | |
| Na2S2O3 x 5 H2O | 1.25 | g | 1.246 | 5.021 | |
| Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
| Na2S x 9 H2O | 0.30 | g | 0.299 | 1.245 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except bicarbonate, pyruvate, thiosulfate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 7.0 - 7.2, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add pyruvate, thiosulfate, vitamins, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of pyruvate and vitamins are sterilized by filtration. | |||||
Media with this solution
| 720 | DESULFITOBACTERIUM HAFNIENSE MEDIUM |