Loading ...

720. DESULFITOBACTERIUM HAFNIENSE MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Hungate tubes Filter Autoclave
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.00 g 0.988 18.473
2 0.10 g 0.099 1.691
3 0.10 g 0.099 0.486
4 0.05 g 0.049 0.336
5 0.40 g 0.395 1.732
6 Trace element solution SL-11 1.00 ml - -
7 Selenite-tungstate solution 1.00 ml - -
8 1.00 g 0.988 -
9
(0.1% w/v)
0.50 ml 4.9e-4 0.002
10 2.60 g 2.569 30.583
11 2.50 g 2.47 22.449
12 1.25 g 1.235 4.977
13 Vitamin solution 10.00 ml - -
14 0.30 g 0.296 1.234
15 1000.00 ml 986.371 54751.8
Instructions:
  1. Dissolve ingredients (except bicarbonate, pyruvate, thiosulfate, vitamins and sulfide), then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add bicarbonate and adjust pH to 7.0 - 7.2, then dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to use add pyruvate, thiosulfate, vitamins, and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas. Stock solutions of pyruvate and vitamins are sterilized by filtration.
Solution occurs in 22 other media (first defined in medium 722 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.2 g 5.2 13.97
2 1.5 g 1.5 7.545
3 70.0 mg 0.07 0.514
4 100.0 mg 0.1 0.505
5 6.0 mg 0.006 0.097
6 190.0 mg 0.19 0.799
7 2.0 mg 0.002 0.012
8 24.0 mg 0.024 0.101
9 36.0 mg 0.036 0.149
10 1000.0 ml 998.207 55408.8
Instructions:
  1. Dissolve EDTA in 800 ml distilled water, adjust pH to 7 using 2 N NaOH and add ferrous chloride. After ferrous chloride has dissolved add other compounds. Finally adjust pH to 6.0 and bring volume to 1000 ml.
Solution occurs in 161 other media (first defined in medium 385 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 12.501
2 3.0 mg 0.003 0.011
3 4.0 mg 0.004 0.012
4 1000.0 ml 998.207 55408.8
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 4
Strains with modifications: 4

Gas composition

  • 100 % N2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaHCO3 2.569 30.583
Na-pyruvate 2.47 22.449
Na2S2O3 x 5 H2O 1.235 4.977
NH4Cl 0.988 18.473
Show more

Cultivation metadata from 4 strains BacDive

pH 6 - 8 1 strain
phyla 1 4 strains
oxygen anaerobe 4 strains
oxygen obligate anaerobe 1 strain
temperature mesophilic 4 strains

Similar media

778b. CALDIMICROBIUM MEDIUM 87 %
507. CARBOXYDOTHERMUS MEDIUM 86 %
793. MOORELLA GLYCERINI MEDIUM 84 %
880. THERMOACETOGENIUM MEDIUM 83 %
292. ACIDAMINOBACTER MEDIUM 82 %

Latest comments

No comments for this medium.