Main sol. 722
Internal Solution-ID: S1473
Equipment needed: Autoclave
Hungate tubes
Filter
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| Na2SO4 | 3.0 | g | 3 | 21.121 | |
| KCl | 0.3 | g | 0.3 | 4.024 | |
| NH4Cl | 0.3 | g | 0.3 | 5.609 | |
| KH2PO4 | 0.2 | g | 0.2 | 1.47 | |
| CaCl2 x 2 H2O | 0.1 | g | 0.1 | 0.68 | |
| NaCl | 50.0 | g | 50 | 855.578 | |
| MgCl2 x 6 H2O | 3.3 | g | 3.3 | 16.232 | |
| MgSO4 x 7 H2O | 1.6 | g | 1.6 | 6.492 | |
| Yeast extract | 0.1 | g | 0.1 | - | |
| Trace element solution SL-11 | 1.0 | ml | - | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na2CO3 | 1.5 | g | 1.5 | 14.153 | |
| Na-DL-lactate | 2.5 | g | 2.5 | 22.31 | |
| Seven vitamins solution | 1.0 | ml | - | - | |
| Na2S x 9 H2O | 0.2 | g | 0.2 | 0.833 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except carbonate, lactate, vitamins and sulfide. Sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic, then dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add lactate, vitamins and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas atmosphere. Vitamins should be sterilized by filtration. The pH of the complete medium should be 7.0 - 7.2. | |||||
Media with this solution
| 722 | OCEANIDESULFOVIBRIO MEDIUM |