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Main sol. 87

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Internal Solution-ID: S151

Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 1.00 g 0.947 -
Glycyl-glycine 1.00 g 0.947 -
Na2HPO4 x 2 H2O 0.10 g 0.095 0.667
MgSO4 x 7 H2O 0.10 g 0.095 0.384
KNO3 0.10 g 0.095 0.937
NaNO3 0.50 g 0.473 5.571
NaCl 0.10 g 0.095 1.62
CaCl2 x 2 H2O 0.05 g 0.047 0.322
Fe(III) citrate
(0.1 g in 100 ml H2O) 		5.00 ml - -
Trace element solution SL-6 1.00 ml - -
Distilled water 1050.00 ml - -
1 Adjust pH to 8.2.
2 Boil the medium under a stream of nitrogen gas for a few minutes and distribute 90 ml medium into 100 ml screw-capped bottles. Bubble each bottle with nitrogen gas and close immediately with a rubber septum and screw tight. Autoclave at 121°C for 15 min. After autoclaving inject 1.0 ml of neutralized sulfide solution (0.03% end concentration) to each bottle. This medium can be stored for several months. Incubate the culture at 50°C at a light intensity of 300 - 500 lux. For heavy cell suspension supplement periodically with sterile yeast extract solution (0.1% end concentration, for more details see Ref. 3365, 3366).
87 CHLOROFLEXUS MEDIUM (modified)