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Vitamin solution

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Internal Solution-ID: S1559

Equipment needed: Autoclave Anaerobic workbench Filter
Compound Amount Unit Conc. [g/L] Conc. [mM]
Vitamin B12 0.1 mg 1.0e-3 7.4e-4
Nicotinic acid 20.0 mg 0.2 1.625
Thiamine-HCl x 2 H2O 10.0 mg 0.1 0.268
p-Aminobenzoic acid 0.2 mg 0.002 0.015
Distilled water 100.0 ml - -
1 Filter sterilise.
765 ISOSPHAERA PALLIDA MEDIUM