MediaDive DSMZ
Content
Media Solutions Ingredients Strains Steps Gas
Search
Medium finder Solution finder Taxonomy search Isolation sources
Tools
Medium builder Unit converter Compare media Compare solutions Prediction SOON
DSMZ
Back to all solutions

Main sol. 778a

Download this solution as

CSV JSON PDF

Internal Solution-ID: S1582

Equipment needed: Filter Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
Na2-9,10-anthraquinone-2,6-disulfonate
(TCI A0308) 		8.25 g 8.225 -
KH2PO4 0.33 g 0.329 2.418
NH4Cl 0.33 g 0.329 6.151
KCl 0.33 g 0.329 4.413
MgCl2 x 6 H2O 0.33 g 0.329 1.618
Trace element solution SL-10 1.00 ml - -
Selenite-tungstate solution 1.00 ml - -
NiCl2 x 6 H2O
(0.1% w/v) 		0.20 ml 2.0e-4 0.002
Yeast extract 1.00 g 0.997 -
NaHCO3 2.50 g 2.493 29.67
CaCl2 x 2 H2O 0.33 g 0.329 2.238
Wolin's vitamin solution (10x) 1.00 ml - -
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except bicarbonate, calcium chloride and vitamins), boil medium for some minutes to dissolve the anthraquinone, then cool under 80% H2 and 20% CO2 gas atmosphere to room temperature. Add solid bicarbonate and adjust pH to 7.2, dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add calcium chloride and vitamins from sterile anoxic stock solutions prepared under 100% N2 gas atmosphere. Vitamins are sterilized by filtration. Adjust pH of complete medium to 7.2, if necessary.
778a THERMOLITHOBACTER MEDIUM